1) Expression Proteomics:
Protein extraction and/or isolation methods.
- For tissues and organs.
- For cultured cells.
- For body fluids.
Isoelectric focusing (1st dimensional running).
Second dimensional running.
Stainings.
Visualization.
Quantitative intensity analysis.
In-gel tryptic digestion.
2) Bioinformatics Analyses:
Peptide mass fingerprinting.
Pearls for bioinformatics analyses of proteins and peptide sequences.
3) Functional Proteomics:
4) Limitations and Pitfalls in Proteomic Analyses: